Polyunsaturated fatty acids (PUFAs) are fatty acids that contain two or more unsaturated double bond structures. Depending on the position of the first unsaturated bond, PUFAs can be divided into ?-3, ?-6, ?-7, ?-9 seri…
Lipids are the basic substances that make up biological membranes. Through lipid-lipid interactions and interactions between lipids and other biomolecules, a complex network of lipid metabolism is formed, which is invol…
Trillions of microorganisms live in the gastrointestinal tract, far outnumbering the body's own cells. The intestinal flora is involved in many physiological and pathological processes in the host, including digestion a…
Metabolomics is a quantitative study of the dynamic patterns of changes in the types and amounts of endogenous small molecule metabolites (generally MW < 1,000) in organisms under the influence of endogenous and exogeno…
Cancer is a complex disease that involves a delicate interplay between the tumor cells and the host immune system. Cytokines play a critical role in this interplay, mediating the communication between different cells of…
Cancer is a complex disease that involves a delicate interplay between the tumor cells and the host immune system. Cytokines play a critical role in this interplay, mediating the communication between different cells of…
Protein immunoaffinity purification coupled with mass spectrometry (IP-MS) can identify numerous potential target protein-interacting proteins. In subsequent studies, it is usually necessary to select a few key interact…
The study of protein-protein interactions is essential for deciphering cellular functions. Various methodologies exist for this purpose, ranging from traditional biochemical assays to advanced proteomic techniques. BioI…
Immunoprecipitation (IP) combined with Western Blot (WB) is a crucial experimental method for studying and validating protein-protein interactions. This article will introduce the experimental procedure of IP-WB and ana…
